Officials said these vehicles were added to the blacklist because their owners failed to pay fines for a long time.
Since the system started, more than 600,000 e-tickets have been issued under the Traffic Regulation and Citation System.
The update was shared during a meeting led by Inspector General Sindh Police Javed Alam Odho, where traffic management and digital ticketing were reviewed.
During the meeting, authorities also decided to launch a strict crackdown on motorcycles without number plates after Eidul Fitr.
This move comes after reports that many motorcyclists are finding ways to avoid the e-challan system.
Also Read: Karachi motorbike riders defeat e-challan system, says DIG Traffic
Traffic officials admitted that some riders have managed to bypass the system, creating challenges for enforcement.
The IG Sindh also took notice of the rising number of traffic accidents in the city and ordered a detailed report on their causes and prevention.
According to rescue data, at least 223 people have died, and more than 2,250 have been injured in traffic accidents in Karachi so far this year.
Many of these accidents involved heavy vehicles like trailers and water tankers, with at least 75 deaths linked to such vehicles.
Authorities said these incidents increased during Ramadan, raising serious safety concerns.
Despite the challenges, officials said traffic arrangements during Ramadan were satisfactory, and efforts are ongoing to improve the system and public convenience.